Intervention of Guizi Decoction on NFAT
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桂枝汤对脾虚大鼠NFAT mRNA，
【摘要】 目的：采用RT-PCR方法来探讨桂枝汤对脾虚NFAT mRNA，IL-4mRNA，IFN-γ mRNA的影响以及干预Th1/Th2细胞漂移的机制。方法：40只大鼠随机分为空白对照组(A组)、脾虚组(B组)、桂枝汤大剂量组(C组)和桂枝汤小剂量组(D组)等4组，每组10只；用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模，B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃，标本采集后用RT-PCR检测NFAT mRNA，IL-4 mRNA和IFN-γ mRNA表达水平。结果：脾虚组的NFAT mRNA，IL-4 mRNA表达上调；而IFN-γ mRNA表达下调，经桂枝汤大、小剂量组治疗后，NFAT mRNA，IL-4 mRNA表达下调，IFN-γ mRNA表达上调。结论：桂枝汤对Th1/Th2细胞漂移作用途径可能是通过下调NFAT mRNA的表达，恢复了IFN-γ mRNA正常转录水平，进而下调IL-4mRNA的表达，使脾虚大鼠Th1/Th2细胞达到一种新的平衡。
Intervention of Guizi Decoction on NFAT mRNA，IL-4mRNA，IFN-γ mRNA in rats with spleen deficiency
【Abstract】AIM: To explore intervention of Guizhi decoction on NFAT mRNA,IL-4mRNA,IFN-γ mRNA and mechanism for Th1/Th2 cell shifting. METHODS: 40 rats were randomly divided into four groups:normal group (group A), asthenic spleen group(group B), high-dosage group (group C) and low-dosage group (group D) with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR. REAULTS: Expression of NFAT mRNA, IL-4mRNA in B group were raised，however, the expression of IFN-γ mRNA were decreased; after treatment, the expression of NFAT mRNA, IL-4mRNA in C group and D group were decreased and expression of IFN-γ mRNA were raised. CONCLUSIONS: Guizhi decoction may interfere Th1/Th2 cell shifting through down-regulating NFAT mRNA, recocering normal transcription of IFN-γmRNA, down-regulating expression of IL-4mRNA,and reaching a new balance of Th1/Th2 cell.
【Key words】 Guizhi decoction; NFAT; IL-4;IFN-γ
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